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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: Jun 8, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
08:01

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

Mutation detection by Southern blotting.

Gillian Mellars1, Keith Gomez

  • 1Katharine Dormandy Haemophilia Centre, Royal Free Hospital, Hampstead, London, UK. g.mellars@medsc.ucl.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2010
PubMed
Summary

The Southern blot, a DNA detection method, revolutionized molecular biology by enabling specific sequence identification. Despite newer techniques, it remains a vital tool for DNA analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • The discovery of DNA structure highlighted the need for DNA sequence identification methods.
  • Edward Southern's 1975 publication introduced a groundbreaking technique for detecting specific DNA fragments.
  • The method's success led to its eponym and inspired related techniques like western and northern blots.

Purpose of the Study:

  • To describe the development and significance of the Southern blot technique.
  • To highlight its role in the identification of DNA sequences.
  • To discuss its enduring importance in molecular biology.

Main Methods:

  • The Southern blot technique involves detecting specific DNA fragments based on sequence complementarity.
  • It utilizes hybridization principles for precise DNA sequence identification.

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Last Updated: Jun 8, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
08:01

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Published on: November 20, 2018

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  • The method has been a standard in molecular biology laboratories for decades.

    • Main Results:

    • The Southern blot became a universally accepted standard for DNA sequence identification.
    • Its simplicity and effectiveness established it as a cornerstone technique.
    • Despite the emergence of faster methods like long-range PCR, it retains its significance.

    Conclusions:

    • The Southern blot, developed in 1975, remains a fundamental technique in molecular biology.
    • Its impact is evident in its name and the development of related blotting techniques.
    • While time-consuming compared to newer methods, its reliability ensures its continued use.