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Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
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Direct induction of CCK and GLP-1 release from murine endocrine cells by intact dietary proteins

Maartje C P Geraedts1, Freddy J Troost, Marc A J G Fischer

  • 1Department of Human Biology, Maastricht University Medical Centre+, Maastricht, The Netherlands. M.Geraedts@HB.unimaas.nl

Molecular Nutrition & Food Research
|October 13, 2010
PubMed
Abstract

Insights

Intact dietary proteins, unlike hydrolyzed forms, significantly stimulate satiety hormones cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1). This suggests intact proteins may be beneficial for weight management strategies.

Area of Science:

  • Gastroenterology and Nutrition Science

Background:

  • High-protein diets are known to increase levels of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1).
  • The precise mechanisms and specific dietary components responsible for this hormonal response remain incompletely understood.

Purpose of the Study:

  • To investigate the differential effects of various dietary proteins, hydrolysates, and synthetic peptides on the secretion of CCK and GLP-1.
  • To identify which specific protein forms are most potent in stimulating these satiety hormones using STC-1 cells.

Main Methods:

  • Utilized STC-1 cells, a model for studying satiety hormone release.
  • Administered intact dietary proteins, protein hydrolysates, and synthetic peptides to STC-1 cells.
  • Measured the subsequent secretion levels of CCK and GLP-1.

Main Results:

  • Intact proteins, including casein, whey, pea, codfish, egg, and wheat, demonstrated significant effects on CCK and GLP-1 release.
  • Egg-hydrolysate notably stimulated both CCK and GLP-1 secretion, while other hydrolysates and synthetic peptides showed minimal impact.
  • Combinations of certain proteins with enzymes (trypsin, DPP-IV) modulated hormone release, with some enhancing CCK and others decreasing GLP-1.

Conclusions:

  • Specific intact or partially digested proteins are potent stimulators of satiety hormone release, more so than protein hydrolysates or synthetic peptides.
  • Intact proteins hold potential as effective dietary supplements for obesity prevention and treatment due to their strong influence on satiety hormones.

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