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Updated: Jun 8, 2026
15:04
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
[A spectrum signals detection method for surface enhanced Raman scattering under high fluorescence and background
Zhi-gang Zhao1, Wei Zhao, Gu Claire
1State Key Laboratory of Electric Power System and Department of Electrical Engineering, Tsinghua University, Beijing 100084, China. zhaothuc@gmail.com
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|October 14, 2010
Summary
A new method effectively detects surface-enhanced Raman scattering (SERS) signals amidst high fluorescence and noise. This technique enhances SERS analysis for applications like biosensing and chemical detection.
Area of Science:
- Spectroscopy
- Analytical Chemistry
- Biophysics
Context:
- Surface-enhanced Raman scattering (SERS) is a powerful technique for molecular detection.
- High fluorescence and background noise often interfere with SERS signal acquisition.
- Accurate detection of SERS signals is crucial for reliable analysis.
Purpose:
- To develop a robust spectrum signal detection method for SERS.
- To address challenges posed by high fluorescence and background noise in SERS spectra.
- To enable precise identification of Raman spectrum peaks.
Summary:
- Analyzed fluorescence and background noise components in SERS signals.
- Evaluated noise components using polynomial and AR models.
- Detected Raman spectrum peaks by analyzing the difference between model evaluation and original data.
Impact:
- Demonstrated high performance and practicability in Rhodamine 6G, prostate-specific antigen, and pH sensing experiments.
- Offers a reliable solution for SERS signal processing in complex environments.
- Enhances the utility of SERS for sensitive and specific molecular detection.
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