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Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
07:52

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Published on: December 21, 2014

Connective tissue growth factor expression pattern in lung development.

Carmen Mesas Burgos1, Magnus Nord, Abraham Roos

  • 1Division for Pediatric Surgery, Astrid Lindgren's Children Hospital, Karolinska Institutet, Stockholm, Sweden. Carmen.mesas.burgos@ki.se

Experimental Lung Research
|October 14, 2010
PubMed
Summary
This summary is machine-generated.

Connective tissue growth factor (CTGF) expression changes during rat lung development. In congenital diaphragmatic hernia (CDH) models, CTGF is decreased, potentially impairing lung growth, while tracheal ligation (TL) increases CTGF, promoting lung development.

Related Experiment Videos

Last Updated: Jun 8, 2026

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
07:52

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Published on: December 21, 2014

Area of Science:

  • Developmental Biology
  • Pulmonology
  • Molecular Biology

Background:

  • Connective tissue growth factor (CTGF) plays a role in tissue development and repair.
  • Congenital diaphragmatic hernia (CDH) is a condition characterized by lung hypoplasia and impaired lung development.
  • Understanding the molecular mechanisms of lung development is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the expression and distribution of CTGF during rat lung development.
  • To compare CTGF patterns in stimulated lung growth (tracheal ligation, TL) and impaired lung growth (nitrofen-induced CDH models).
  • To elucidate CTGF's potential role in lung development and CDH pathogenesis.

Main Methods:

  • Used Sprague-Dawley rat fetuses at gestational days E14, E17, and E21.
  • Induced CDH model using nitrofen and stimulated lung growth model using tracheal ligation (TL).
  • Performed immunohistochemical analysis for CTGF protein and real-time PCR for CTGF mRNA expression.

Main Results:

  • CTGF expression increased during normal lung development, localized to the epithelium of terminal bronchioles.
  • In nitrofen-induced CDH lungs, CTGF protein was decreased in distal airway epithelium and increased in mesenchyme.
  • In TL-induced hyperplastic lungs, CTGF expression was increased, particularly in terminal bronchiolar epithelium, and mRNA levels were elevated.
  • Conversely, CTGF mRNA levels were decreased in nitrofen-induced CDH lungs.

Conclusions:

  • This study provides the first report on CTGF expression patterns during lung development and in CDH models.
  • Decreased CTGF expression in CDH lungs may contribute to impaired alveologenesis and abnormal microvascular development.
  • Increased CTGF expression in TL lungs suggests a role in promoting lung growth, offering potential therapeutic insights for conditions like CDH.