Related Experiment Video
Updated: Jun 8, 2026

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines
Published on: February 25, 2013
Mouse spermatogonial stem cells obtain morphologic and functional characteristics of hematopoietic cells in vivo
Liang Ning1, Ellen Goossens, Mieke Geens
1Research Group Biology of the Testis (BITE), Department of Embryology and Genetics (EMGE), Vrije Universiteit Brussel, Brussels, Belgium. lianning@vub.ac.be
Background:
The aim of this study was to explore the plasticity and transdifferentiation potential of murine spermatogonial stem cells (SSCs) into hematopoietic cells.
Methods:
GFP(+)CD49f(+)H-2K(b-) SSCs of male donor mice were isolated and injected into the bone marrow (BM) of Busulfan-treated GFP(-) female mice. Twelve weeks post-transplantation, the recipients were sacrificed and their BM, peripheral blood (PB) and spleen (SL) cells were collected and evaluated by phenotypical methods, i.e. fluorescence-activated cell sorting, immunohistochemistry and fluorescence in situ hybridization, and functional assays, i.e. colony-forming units assay and intra-BM transplantation.
Results:
Green fluorescent protein (GFP)- and Y chromosome-positive cells were observed in the BM, PB and SL of transplanted female mice. These cells presented phenotypical and functional characteristics of hematopoietic cells in vitro and in vivo.
Conclusions:
Our results indicate that SSCs have the potential to transdifferentiate into hematopoietic cells in vivo.
Related Concept Videos
Spermatogenesis
Spermatogenesis
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
Multipotency of Hematopoietic Stem Cells
Mesenchymal Stem Cells
Hematopoiesis
Source And Potency Of Stem Cells

