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Related Experiment Video

Updated: Jun 8, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

TGF-ß1 and FAK regulate periostin expression in PDL fibroblasts.

W Wen1, E Chau, L Jackson-Boeters

  • 1Division of Oral Biology, Schulich School of Medicine and Dentistry, Dental Sciences Building, The University of Western Ontario, London, Ontario, Canada.

Journal of Dental Research
|October 14, 2010
PubMed
Summary

Periostin protein is present in human periodontal ligaments (PDL) and its expression is influenced by mechanical forces and growth factors. Transforming growth factor-beta1 (TGF-β1) regulates periostin via focal adhesion kinase (FAK) pathways.

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Last Updated: Jun 8, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Periodontal Research

Background:

  • Periostin is crucial for collagen formation and fibroblast differentiation in murine periodontal ligaments (PDL).
  • Understanding periostin's role in human PDL is essential for periodontal health and regeneration.
  • Investigating the regulation of periostin in human PDL fibroblasts is key to elucidating its function.

Purpose of the Study:

  • To determine the in situ expression of periostin protein in the human PDL.
  • To explore the in vitro mechanisms regulating periostin expression in human PDL fibroblasts.
  • To elucidate the roles of mechanical strain and TGF-β1 in periostin regulation.

Main Methods:

  • Immunohistochemistry was used to detect periostin protein in human PDL.
  • Human PDL fibroblasts were subjected to in vitro cyclic strain and TGF-β1 treatment.
  • Focal adhesion kinase (FAK) inhibition and FAK-null fibroblasts were utilized to study signaling pathways.

Main Results:

  • Periostin protein was detected in the human PDL, with lower expression in teeth under reduced occlusal loading.
  • Cyclic strain and TGF-β1 significantly increased periostin mRNA levels in PDL fibroblasts.
  • FAK signaling was essential for basal and stimulated periostin mRNA expression.

Conclusions:

  • Periostin is strongly expressed in the human PDL.
  • Mechanical strain and TGF-β1 modulate periostin mRNA levels in human PDL fibroblasts.
  • FAK-dependent pathways are critical for regulating periostin expression.