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Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

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A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Wide spectral range confocal microscope based on endlessly single-mode fiber.

R Hubbard¹, Yu B Ovchinnikov, J Hayes

¹National Physical Laboratory, Hampton Road, Teddington, Middlesex, TW11 0LW, UK.

|October 14, 2010

Summary

This summary is machine-generated.

This study introduces a novel fiber-optic confocal microscope using photonic crystal fiber for broad spectral range imaging. It achieves high resolution, enabling visualization of single photons from quantum dots.

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Area of Science:

Optics and Photonics
Microscopy
Quantum Dot Imaging

Background:

Confocal microscopy is a powerful imaging technique.
Fiber-optic microscopy offers portability and flexibility.
Photonic crystal fibers (PCFs) enable novel optical properties.

Purpose of the Study:

To develop a single-mode, fiber-optic confocal microscope.
To confine broad spectral ranges within a single spatial mode.
To demonstrate imaging of quantum emitters.

Main Methods:

Utilized a large mode area photonic crystal fiber.
Achieved single-mode operation over an optical octave.
Measured resolution at 1.0 μm lateral and 2.5 μm axial at 900 nm.

Main Results:

Demonstrated endlessly single-mode operation.
Confined broad spectral excitation and emission wavelengths to a single spatial mode.
Successfully imaged single photons from individual InAs quantum dots.

Conclusions:

The developed fiber-optic confocal microscope offers high resolution and broad spectral compatibility.
This technology is suitable for imaging quantum emitters and other nanoscale phenomena.
Potential applications in quantum optics, materials science, and biotechnology.