Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)
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Updated: Jun 8, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
Published on: February 24, 2026
Thomas Dertinger1, Ryan Colyer, Robert Vogel
1Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, California, USA. t.dertinger@chem.ucla.edu
Abstract:
Superresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Point-Spread Function. We show a two-fold increase of resolution (over the diffraction limit) of near-infrared quantum dot labeled tubulin-network of 3T3 fibroblasts.
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