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Updated: Jun 8, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Wavefield back-propagation in high-resolution X-ray holography with a movable field of view
Erik Guehrs1, Christian M Günther, Bastian Pfau
1Institut für Optik und Atomare Physik, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin, Germany. erik.guehrs@tu-berlin.de
Abstract:
Mask-based Fourier transform holography is used to record images of biological objects with 2.2 nm X-ray wavelength. The holography mask and the object are decoupled from each other which allows us to move the field of view over a large area over the sample. Due to the separation of the mask and the sample on different X-ray windows, a gap between both windows in the micrometer range typically exists. Using standard Fourier transform holography, focussed images of the sample can directly be reconstructed only for gap distances within the setup's depth of field. Here, we image diatoms as function of the gap distance and demonstrate the possibility to recover focussed images via a wavefield back-propagation technique. The limitations of our approach with respect to large separations are mainly associated with deviations from flat-field illumination of the object.
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