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Updated: Jun 8, 2026

Imaging Subcellular Structures in the Living Zebrafish Embryo
Published on: April 2, 2016
Twin-photon confocal microscopy
1Dept of Electrical and Computer Engineering, Boston University, 8 Saint Mary’s St, Boston, MA 02215, USA. simond@bu.edu
Abstract:
A recently introduced two-channel confocal microscope with correlated detection promises up to 50% improvement in transverse spatial resolution [Simon, Sergienko, Optics Express 18, 9765 (2010)] via the use of photon correlations. Here we achieve similar results in a different manner, introducing a triple-confocal correlated microscope which exploits the correlations present in optical parametric amplifiers. It is based on tight focusing of pump radiation onto a thin sample positioned in front of a nonlinear crystal, followed by coincidence detection of signal and idler photons, each focused onto a pinhole. This approach offers further resolution enhancement in confocal microscopy.
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