Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of Saccharomyces cerevisiae fermentation product on intestinal health and productivity of coccidian-challenged laying hens.

Poultry science·2012
Same author

Rigid endoscopic relay systems: a comparative study.

Applied optics·2010
Same author

Control of the radial gradient-index profile by leaching of a gel.

Applied optics·2010
Same author

Evaluation of XPC and prototypes on aflatoxin-challenged broilers.

Poultry science·2010
Same author

Effects of axial and radial gradients on Cooke triplets.

Applied optics·2010
Same author

Design and tolerancing of aspherical and gradient-index germanium singlets of equal performance.

Applied optics·2010
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Quantitative phase imaging in confocal microscopy by optical differentiation.

A W Kulawiec, D T Moore

    Applied Optics
    |October 14, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Optical differentiation in confocal microscopy enables quantitative phase imaging. This technique uses specialized filters and signal processing to reconstruct detailed surface profiles, advancing microscopy applications.

    More Related Videos

    Quantitative Immunofluorescence to Measure Global Localized Translation
    09:13

    Quantitative Immunofluorescence to Measure Global Localized Translation

    Published on: August 22, 2017

    Phase Contrast and Differential Interference Contrast (DIC) Microscopy
    06:49

    Phase Contrast and Differential Interference Contrast (DIC) Microscopy

    Published on: August 6, 2008

    Related Experiment Videos

    Last Updated: Jun 8, 2026

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    Quantitative Immunofluorescence to Measure Global Localized Translation
    09:13

    Quantitative Immunofluorescence to Measure Global Localized Translation

    Published on: August 22, 2017

    Phase Contrast and Differential Interference Contrast (DIC) Microscopy
    06:49

    Phase Contrast and Differential Interference Contrast (DIC) Microscopy

    Published on: August 6, 2008

    Area of Science:

    • Optical Physics
    • Microscopy
    • Image Processing

    Background:

    • Quantitative phase imaging (QPI) is crucial for label-free microscopy.
    • Traditional QPI methods can be complex or limited in resolution.
    • Confocal microscopy offers high spatial resolution but typically lacks phase information.

    Purpose of the Study:

    • To develop a novel method for quantitative phase imaging using optical differentiation.
    • To adapt this technique for confocal microscopy systems.
    • To demonstrate its utility in surface profiling applications.

    Main Methods:

    • Applying optical differentiation principles to confocal microscopy.
    • Incorporating one-dimensional absorptive filters into the microscope's pupil.
    • Utilizing signal processing and integration to derive phase information from generated images.

    Main Results:

    • Successfully generated images related to the local phase slope of the object.
    • Achieved quantitative phase profiles through signal processing and integration.
    • Demonstrated the technique's effectiveness in a reflection-based surface-profiling instrument.

    Conclusions:

    • Optical differentiation is a viable technique for quantitative phase imaging in confocal microscopy.
    • This method allows for the reconstruction of quantitative phase profiles.
    • The demonstrated surface-profiling instrument showcases the practical application of this advanced imaging approach.