Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanoscale tilt measurement using a cyclic interferometer with polarization phase stepping and multiple reflections.

Applied optics·2018
Same author

In vitro glucuronidation of the antibacterial triclocarban and its oxidative metabolites.

Drug metabolism and disposition: the biological fate of chemicals·2011
Same author

Dual-wavelength heterodyne differential interferometer for high-precision measurements of reflective aspherical surfaces and step heights.

Applied optics·2010
Same author

Theoretical analysis of confocal microscopy with microlenses.

Applied optics·2010
Same author

Absolute interferometric distance measurement using a FM-demodulation technique.

Applied optics·2010
Same author

Semispatial, robust, and accurate phase evaluation algorithm.

Applied optics·2010

Related Experiment Video

Updated: Jun 8, 2026

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
12:14

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

Published on: August 12, 2013

Oblique incidence and observation electronic speckle-pattern interferometry.

C Joenathan, B Franze, H J Tiziani

    Applied Optics
    |October 14, 2010
    PubMed
    Summary

    This study introduces an electronic speckle-pattern interferometer using an anamorphic prism for oblique incidence. This setup enables collinear object and reference beams for enhanced interferometric measurements.

    More Related Videos

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    Related Experiment Videos

    Last Updated: Jun 8, 2026

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    Area of Science:

    • Optical Engineering
    • Metrology
    • Interferometry

    Background:

    • Electronic speckle-pattern interferometry (ESPI) is a non-contact optical measurement technique.
    • Traditional ESPI setups often require complex optical arrangements for oblique incidence and observation.
    • Achieving phase stepping in ESPI can enhance measurement accuracy and facilitate quantitative analysis.

    Purpose of the Study:

    • To present a novel electronic speckle-pattern interferometer design.
    • To utilize an anamorphic prism for oblique incidence and observation.
    • To introduce a phase-stepping capability for improved measurement.

    Main Methods:

    • An anamorphic prism is integrated into the interferometer setup.
    • A collimated beam is directed through the prism, with partial reflection forming the reference beam.
    • Transmitted light illuminates the object, creating the object beam, with both beams being collinear.
    • A modified scheme using two prisms is proposed for phase stepping.

    Main Results:

    • The proposed design simplifies the optical path by making object and reference beams collinear.
    • The use of an anamorphic prism facilitates oblique incidence and observation.
    • A new two-prism scheme enables phase stepping within the interferometer.

    Conclusions:

    • The developed interferometer offers a more straightforward configuration for oblique incidence measurements.
    • The integration of phase stepping enhances the quantitative capabilities of the system.
    • This approach advances the application of ESPI in various fields requiring precise surface deformation analysis.