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Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers
Published on: June 30, 2018
Enzyme-functionalized polymer brush films on the inner wall of silicon-glass microreactors with tunable biocatalytic
Francesca Costantini1, Edmondo M Benetti, David N Reinhoudt
1Molecular Nanofabrication (MnF), University of Twente, MESA+ Institute for Nanotechnology, P.O. Box 217, 7500, AE, Enschede, The Netherlands.
Abstract:
The lipase from Candida Rugosa was immobilized to a poly(methacrylic acid) polymer brush layer, grown on the inner wall of silicon-glass microreactors. The hydrolysis of 4-nitrophenyl acetate was used as a model reaction to study the activity of this biocatalytic system. The amount of bound lipase could be tuned by changing the polymerization time of the brush formation. The Michaelis-Menten constants and V(max) values, determined for immobilized and free lipase, are similar, demonstrating that the lipase's substrate affinity and its activity remain unchanged upon immobilization to the microchannel wall.
