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Updated: Jun 8, 2026

Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
Published on: June 8, 2022
Bacteria competing with the adhesion and biofilm formation by Staphylococcus aureus
B Sadowska1, E Walencka, M Wieckowska-Szakiel
1Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, 90-237 Łódź, Poland.
Abstract:
The activity of antagonistic substances produced by Pseudomonas aeruginosa and Lactobacillus acidophilus against the planktonic and sessile populations of Staphylococcus aureus strains was demonstrated. The strongest effects were caused by probiotic L. acidophilus strain - bacteriocin-like inhibitory substances (BLIS) positive. However, the S. aureus A3 growth, adhesion and biofilm formation was also limited by cell-free supernatant of L. acidophilus H-1 (BLIS negative). Moreover, competitive direct interactions were observed between staphylococci and the above bacteria, which influenced the formation of dualspecies aggregates on the surface.
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