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Bone Marrow-derived Macrophage Production
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Published on: November 22, 2013

Human regulatory macrophages

James A Hutchinson1, Paloma Riquelme, Edward K Geissler

  • 1Laboratory for Transplantation Research, Department of Surgery, University Hospital Regensburg, Regensburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|October 14, 2010
PubMed

Regulatory macrophages (M regs) are a novel type of suppressor macrophage which may be a particularly suitable cell for inducing tolerance of solid organ transplants. In this article, we provide a detailed description of the generation of human M regs from peripheral blood monocytes and methods for the assessment of their phenotype. The uniqueness of the human M reg is best appreciated when the M reg is compared to macrophages in other states of activation; therefore, protocols are provided for generating five comparator macrophage types which have been used as cell type-specificity controls in our work.

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