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Related Experiment Videos

The mouse spleen white pulp response to continuous hypoxia. A digital image processing analysis.

A C Nessi de Aviñón1, M C Bengtsson

  • 1Instituto de Biología Celular, Facultad de Medicina, UBA, Buenos Aires, Argentina.

Acta Physiologica Et Pharmacologica Latinoamericana : Organo De La Asociacion Latinoamericana De Ciencias Fisiologicas Y De La Asociacion Latinoamericana De Farmacologia
|January 1, 1990
PubMed
Summary

Mice spleen white pulp surface exhibits a 24-hour rhythm. Hypoxia alters this pattern, leading to a smaller spleen size in treated animals compared to controls.

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Area of Science:

  • Physiology
  • Immunology
  • Chronobiology

Background:

  • The spleen plays a crucial role in immune responses and hematopoiesis.
  • Circadian rhythms influence various physiological processes, including immune function.
  • Understanding spleen's rhythmic variations is key to comprehending its overall function.

Purpose of the Study:

  • To investigate the 24-hour periodicity of spleen white pulp surface in CFW mice.
  • To determine the impact of hypoxia on this rhythmic pattern and spleen size.

Main Methods:

  • Utilized 90-day-old male CFW mice under standard conditions.
  • Monitored spleen white pulp surface area in cross-sections over time.
  • Administered continuous hypoxia treatment for a defined period.

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  • Quantified size variations using pixel counts, convertible to square micrometers.

    • Main Results:

    • A distinct 24-hour variation pattern was observed in the spleen white pulp surface.
    • Hypoxia significantly modified this pattern within the initial 18 hours of treatment.
    • A new steady state was established after 18 hours of hypoxia.
    • Spleens from hypoxic mice were consistently smaller than those of control mice.

    Conclusions:

    • The spleen white pulp surface exhibits circadian rhythmicity.
    • Hypoxia disrupts normal spleen periodicity and leads to a reduction in spleen size.
    • Observed size variations are real physiological changes, not artifacts.