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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.

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Related Experiment Video

Updated: Jun 8, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Solid-state NMR spectroscopy on complex biomolecules.

Marie Renault1, Abhishek Cukkemane, Marc Baldus

  • 1Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Angewandte Chemie (International Ed. in English)
|October 14, 2010
PubMed
Summary
This summary is machine-generated.

Solid-state NMR (ssNMR) spectroscopy offers atomic-level insights into challenging biomolecular systems. Recent advancements expand its use in structural and cellular biology, overcoming previous limitations.

Related Experiment Videos

Last Updated: Jun 8, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Area of Science:

  • Biophysics
  • Structural Biology
  • Molecular Biology

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is traditionally linked to soluble molecules and MRI.
  • Solid-state NMR (ssNMR) spectroscopy has provided atomic-level insights into complex biomolecular systems since the 1970s.
  • Lipid bilayers and biomaterials are examples of systems studied using ssNMR.

Purpose of the Study:

  • To review current approaches and challenges in ssNMR for biomolecular studies.
  • To highlight recent progress in ssNMR applications at the interface of structural and cellular biology.

Main Methods:

  • Solid-state NMR (ssNMR) spectroscopy.
  • Advancements in NMR spectroscopy, biophysics, and molecular biology.

Main Results:

  • ssNMR spectroscopy provides atomic-level insight into complex biomolecular systems.
  • Recent progress has significantly expanded the repertoire of ssNMR for biomolecular studies.
  • ssNMR is increasingly used at the interface of structural and cellular biology.

Conclusions:

  • ssNMR is a powerful technique for studying insoluble and complex biomolecular systems.
  • Continued progress in methodology and interdisciplinary collaboration enhances ssNMR capabilities.
  • ssNMR plays a vital role in advancing structural and cellular biology research.