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Phylogeny of Pilobolaceae
K Michael Foos1, Nicole L May, Dale L Beach
1School of Natural Science and Mathematics, Indiana University East, Richmond, Indiana 47374, USA. foos@iue.edu
Mycologia
|October 15, 2010
Summary
Phylogenetic analysis using ribosomal DNA (rDNA) sequences reveals Pilaira should be removed from Pilobolaceae. Traditional fungal morphology is insufficient for classifying Pilobolus species.
Area of Science:
- Mycology
- Molecular Systematics
- Fungal Phylogenetics
Background:
- Traditional fungal classification relies on morphological characteristics.
- The Zygomycota phylum has limited genetic sequence data for classification.
- Pilobolaceae genera (Pilaira, Pilobolus, etc.) lack distinct morphological differences.
Purpose of the Study:
- To investigate the phylogenetic relationships within the Pilobolaceae family.
- To determine if molecular data can resolve taxonomic ambiguities in Pilobolaceae.
- To re-evaluate the classification of Pilaira and Pilobolus based on genetic evidence.
Main Methods:
- DNA sequencing of three ribosomal RNA (rRNA) gene regions.
- Phylogenetic analysis using molecular data.
- Comparison of molecular phylogenies with traditional morphological characteristics.
Main Results:
- Molecular phylogenetic analysis indicates Pilaira is not closely related to Pilobolus, suggesting its removal from Pilobolaceae.
- Ribosomal DNA (rDNA) clades correlate strongly with sporangiospore size.
- Morphological traits alone are inadequate for differentiating species within Pilobolus.
Conclusions:
- The Pilobolaceae family requires redescription following the exclusion of Pilaira.
- Molecular phylogenetic data is crucial for accurate fungal classification, especially within the Zygomycota.
- Sporangiospore size is a key morphological feature that aligns with genetic classifications.
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