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Updated: Jun 8, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Two putative RNA-binding proteins function with unequal genetic redundancy in the MOS4-associated complex.

Jacqueline Monaghan¹, Fang Xu, Shaohua Xu

¹Michael Smith Laboratories and Department of Botany, University of British Columbia, Vancouver, Canada.

Plant Physiology

|October 15, 2010

Summary

The MOS4-associated complex (MAC) includes MAC5A, an RNA-binding protein vital for plant immunity. While MAC5A and its homolog MAC5B show partial redundancy, their combined absence is lethal, impacting plant autoimmune responses.

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Last Updated: Jun 8, 2026

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Published on: August 9, 2019

Area of Science:

Plant molecular biology
Nuclear protein complexes
RNA-binding proteins

Background:

The MOS4-associated complex (MAC) is a conserved nuclear complex linked to the spliceosome.
Previous research identified core MAC proteins essential for plant defense responses.

Purpose of the Study:

To characterize MAC5A and its homolog MAC5B, identified as components of the Arabidopsis MAC.
To investigate the function of MAC5A and MAC5B in plant immunity and development.

Main Methods:

Co-immunoprecipitation to confirm MAC5A's association with the MAC.
Green Fluorescent Protein (GFP) tagging for subcellular localization studies.
Double mutant analysis to assess genetic redundancy and developmental effects.

Main Results:

MAC5A was confirmed as a component of the Arabidopsis MAC and localizes to the nucleus.
MAC5A and MAC5B exhibit unequal genetic redundancy, with a double mutant (mac5a mac5b) being lethal.
Single mutants (mac5a, mac5b) did not show altered susceptibility to pathogens.
The mac5a-1 mutation partially suppressed the autoimmune phenotype of the snc1 mutant.

Conclusions:

MAC5A is a nuclear component of the MAC involved in regulating plant autoimmune responses.
The study highlights the importance of MAC5A and MAC5B in plant development and immunity, particularly in the context of autoimmunity.