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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Novel multiplex PCR using dual-priming oligonucleotides for detection and discrimination of the Mycobacterium
Hye Ryun Lee1, So Yeon Kim, Ho Eun Chang
1Department of Laboratory Medicine, Seoul National University Bundang Hospital, 463-707, Gumi-dong, Seongnam-si, Bundang-gu, Gyeonggi-do, South Korea.
Journal of Clinical Microbiology
|October 15, 2010
Abstract:
We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species.
