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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus

Surbhi Malhotra-Kumar¹, Liesbet Van Heirstraeten, Andie Lee

¹Department of Medical Microbiology, Vaccine & Infectious Disease Institute, Campus Drie Eiken, University of Antwerp, S3, Universiteitsplein 1, B-2610 Wilrijk, Antwerp, Belgium. surbhi.malhotra@ua.ac.be

Journal of Clinical Microbiology

|October 15, 2010

Abstract:

The diagnostic sensitivities of the BD GeneOhm and Cepheid Xpert assays were compared using culture on log-serial dilutions of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and non-MRSA strains and on nasal and groin swabs from patients with histories of MRSA carriage. The sensitivities of GeneOhm and Xpert were high at 10(3)-CFU/ml MRSA concentrations (92.3% and 96.3%, respectively) although decreased considerably (<35%) at a 1-log-lower concentration. Unexpectedly, both assays also detected select coagulase-negative staphylococci, which requires further evaluation.

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