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A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Identification of blue-light photoresponse genes in oyster mushroom mycelia
Yoko Nakano1, Hiroshi Fujii, Masanobu Kojima
1Interdisciplinary Graduate School of Science and Technology, Shinshu University, Kamiina, Nagano, Japan.
Abstract:
Using the characteristics of light-emitting diodes, we studied the effects of light quality, light intensity, and repeated light-dark cycles on the growth of oyster mushroom mycelia. Blue-light stimulation suppressed growth in oyster mushroom mycelia, and this effect was remarkably dependent on light intensity. Random amplified polymorphic DNA differential display analysis enabled the identification for the first time of 15 upregulated and 13 downregulated genes induced by blue-light stimulation. On the basis of a homologous search using BLASTX and the Interpro database, the transcription products were analyzed to identify the putative proteins. In addition, by real-time PCR analysis, the time courses of the expression levels of eight of the genes identified were found to include intrinsic induction time.
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