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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 8, 2026

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
06:28

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Published on: October 1, 2015

Direct and indirect immunofluorescence.

Valéria Aoki1, Joaquim X Sousa, Lígia M I Fukumori

  • 1Department of Dermatology, University of Sao Paulo, Sao Paulo, Brazil. valaoki@hotmail.com

Anais Brasileiros De Dermatologia
|October 15, 2010
PubMed
Summary
This summary is machine-generated.

Immunofluorescence is a valuable diagnostic tool for skin diseases when other methods are inconclusive. This laboratory technique detects immune deposits crucial for understanding the pathogenesis of autoimmune bullous and inflammatory disorders.

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Last Updated: Jun 8, 2026

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
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Published on: January 17, 2025

Area of Science:

  • Dermatology
  • Immunology
  • Pathology

Background:

  • Clinical and histopathologic findings can be inconclusive for diagnosing certain skin diseases.
  • Autoimmune bullous diseases and inflammatory disorders present diagnostic challenges.

Purpose of the Study:

  • To highlight the utility of immunofluorescence as a diagnostic tool.
  • To explain the role of immunofluorescence in identifying immune deposits in skin diseases.

Main Methods:

  • Immunofluorescence assays.
  • Detection of in situ and circulating immune deposits.
  • Requires experienced laboratory technicians.

Main Results:

  • Immunofluorescence serves as a valuable auxiliary diagnostic method.
  • It effectively detects immune deposits involved in disease pathogenesis.

Conclusions:

  • Immunofluorescence is a feasible and important laboratory method for diagnosing autoimmune bullous and inflammatory skin diseases.
  • It aids in understanding the immunopathogenesis of these conditions.