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Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
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Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

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Protease activation of split green fluorescent protein

Brian P Callahan1, Matthew J Stanger, Marlene Belfort

  • 1Wadsworth Center, NYS Department of Health, Albany, NY 12201-2002, USA.

Chembiochem : a European Journal of Chemical Biology
|October 15, 2010
PubMed
Summary

No abstract available in PubMed .

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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