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Micropatterned Magneto-Rheological Elastomers to Drive Changes in Cardiomyocyte Alignment
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Scaffold stiffness affects the contractile function of three-dimensional engineered cardiac constructs.

Anna Marsano1, Robert Maidhof, Leo Q Wan

  • 1Dept. of Biomedical Engineering, Columbia University, New York, NY 10032, USA.

Biotechnology Progress
|October 15, 2010
PubMed
Summary

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Low stiffness in poly(glycerol sebacate) scaffolds enhances cardiomyocyte function. This study shows that softer scaffolds promote better tissue formation and contractile performance in engineered cardiac constructs.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cardiovascular Research

Background:

  • Optimizing scaffold properties is crucial for developing functional engineered cardiac tissues.
  • Poly(glycerol sebacate) (PGS) is a promising elastomer for cardiac tissue engineering due to its tunable properties.

Purpose of the Study:

  • To investigate the impact of initial scaffold stiffness on cardiomyocyte assembly and function.
  • To evaluate the role of laminin coating in improving cell seeding and construct development.

Main Methods:

  • Cultured cardiomyocytes on three-dimensional poly(glycerol sebacate) scaffolds with varying stiffness (low, medium, high kPa).
  • Utilized perfusion culture for 8 days and assessed construct properties, including cell density, contractility, and matrix deposition.

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Micropatterned Magneto-Rheological Elastomers to Drive Changes in Cardiomyocyte Alignment
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Micropatterned Magneto-Rheological Elastomers to Drive Changes in Cardiomyocyte Alignment

Published on: June 10, 2025

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
12:13

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization

Published on: October 28, 2013

  • Applied laminin coating to evaluate its effect on cell seeding efficiency.

    • Main Results:

    • Laminin coating significantly improved cell seeding efficiency (59% to 90%) and subsequent construct development.
    • Low and medium stiffness scaffolds supported compact tissue formation, while high stiffness did not.
    • The low stiffness group exhibited superior contraction amplitude and higher final compressive modulus.

    Conclusions:

    • Scaffold stiffness is a critical factor influencing the functional assembly of engineered cardiac constructs.
    • Lower compressive stiffness in poly(glycerol sebacate) scaffolds correlates positively with enhanced contractile function.
    • Laminin coating improves cell-material interactions, promoting better tissue development.