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Related Experiment Video

Updated: Jun 8, 2026

Protocol to Create Chronic Wounds in Diabetic Mice
06:55

Protocol to Create Chronic Wounds in Diabetic Mice

Published on: September 25, 2019

Macroscale spatial variation in chronic wound microbiota: a cross-sectional study.

Lance B Price1, Cindy M Liu, Yelena M Frankel

  • 1Center for Microbiomics and Human Health, Translational Genomics Research Institute, Flagstaff, Arizona 86001, USA. lprice@tgen.org

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society
|October 16, 2010
PubMed
Summary
This summary is machine-generated.

Chronic wound investigations benefit from controlling sample sites. Bacterial communities within individual wounds show significant similarity, suggesting composite sampling for robust microbiota characterization.

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Area of Science:

  • Microbiology
  • Wound Healing
  • Genomics

Background:

  • Microbiological investigations of chronic wounds often control for sample site.
  • Macroscale spatial variation in chronic wound microbiota is not well understood.

Purpose of the Study:

  • To characterize the macroscale spatial variation of bacterial communities within chronic wounds.
  • To evaluate the impact of sample site on chronic wound microbiota studies.

Main Methods:

  • Collected 31 curette samples from 13 chronic wounds at different sites (leading edge, center).
  • Utilized 16S rRNA gene pyrosequencing for bacterial community analysis.
  • Employed statistical methods including hierarchical clustering and PERMANOVA.

Main Results:

  • Identified 58 bacterial families and 91 genera across the wounds.
  • Observed significant macroscale spatial variation among different wounds.
  • Found bacterial communities within individual wounds were more similar to each other than to those in different wounds (p=0.001).

Conclusions:

  • Controlling for sample site can enhance the quality of chronic wound microbiota studies.
  • Studies not controlling for sample site should not be dismissed solely on this basis.
  • Composite sampling from multiple wound sites may offer the most comprehensive microbiota characterization.