Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GLP-1R and GIPR crosstalk modulates insulinotropic signaling pathways.

Cell chemical biology·2026
Same author

Proteomic analysis of dental enamel from 20 Homo naledi individuals shows no male markers.

Cell·2026
Same author

Deciphering cytokine-driven ADP-ribosylation signaling networks via Af1521-based mass spectrometry analysis of labile Glu/Asp-linkages.

Nature communications·2026
Same author

The proteomics and phosphoproteomics landscape of melanoma under T cell attack.

Cell reports. Medicine·2026
Same author

Glucocorticoid-Induced Proteome and Phosphoproteome Changes in Breast Cancer Cell Lines.

Journal of proteome research·2026
Same author

Quantitative proteomics and phosphoproteomics reveal glucocorticoid stimulation of TLR and Rho GTPase signaling in neutrophil-like cells.

Genome biology·2026

Related Experiment Video

Updated: Jun 8, 2026

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
08:43

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

Published on: August 21, 2009

Yeast expression proteomics by high-resolution mass spectrometry.

Tobias C Walther1, Jesper V Olsen, Matthias Mann

  • 1Organelle Architecture and Dynamics, Max Planck Institute of Biochemistry, Martinsried, Germany.

Methods in Enzymology
|October 16, 2010
PubMed
Summary

Global protein quantification in yeast is now possible using advanced mass spectrometry and bioinformatics. This allows for a more accurate understanding of cellular processes beyond RNA levels, crucial for studying stress responses.

More Related Videos

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
08:43

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

Published on: August 21, 2009

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

  • Proteomics
  • Yeast as a model organism
  • Systems biology

Background:

  • RNA abundance measurements are common but don't always reflect protein levels.
  • Proteins drive biological functions, and their abundance is regulated by translation and post-translation.
  • Accurate protein quantification is essential for understanding cellular mechanisms, especially during stress.

Purpose of the Study:

  • To provide background on mass spectrometry-based proteomics.
  • To describe the methodology for comprehensive yeast proteome analysis.
  • To highlight the importance of global protein quantification in yeast.

Main Methods:

  • Development of new sample preparation techniques.
  • High-resolution mass spectrometry for protein identification and quantification.
  • Novel bioinformatics tools for data analysis.

Main Results:

  • Enables global quantitation of the yeast proteome.
  • Provides a more accurate picture of cellular composition than RNA analysis alone.
  • Facilitates the study of protein abundance regulation under various conditions.

Conclusions:

  • Global proteome analysis in yeast is now feasible.
  • This approach is vital for understanding biological processes and stress responses.
  • Advances in proteomics are closing the gap between gene expression and protein function.