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Related Experiment Video

Updated: Jun 8, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
07:00

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Published on: June 14, 2013

Imaging single mRNA molecules in yeast.

Hyun Youk1, Arjun Raj, Alexander van Oudenaarden

  • 1Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Methods in Enzymology
|October 16, 2010
PubMed
Summary
This summary is machine-generated.

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Cellular variation is common. This study introduces a method to visualize single mRNA molecules in individual yeast cells, enabling detailed analysis of cellular differences.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Isogenic yeast populations exhibit phenotypic heterogeneity.
  • Population-averaged measurements obscure individual cell behavior.
  • Understanding single-cell variation is crucial for biological insights.

Purpose of the Study:

  • To present a method for analyzing cellular variation at the single-molecule level.
  • To enable visualization of individual mRNA molecules within fixed yeast cells.
  • To facilitate the study of gene expression heterogeneity.

Main Methods:

  • Developed a protocol based on fluorescence in situ hybridization (FISH).
  • Adapted FISH for visualizing single mRNA molecules.
  • Applied the technique to fixed yeast cells.

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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Published on: May 25, 2018

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
09:52

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

Published on: September 15, 2020

Related Experiment Videos

Last Updated: Jun 8, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
07:00

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Published on: June 14, 2013

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
09:28

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Published on: May 25, 2018

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
09:52

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

Published on: September 15, 2020

Main Results:

  • Successfully rendered single mRNA molecules visible in individual yeast cells.
  • Demonstrated the capability to measure molecular events within single cells.
  • Provided a foundation for quantitative analysis of cellular heterogeneity.

Conclusions:

  • The described FISH technique allows for the study of molecular variation in individual cells.
  • This method overcomes limitations of population-averaged measurements.
  • Enables deeper understanding of cellular heterogeneity in yeast.