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Related Experiment Video

Updated: Jun 8, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Visualizing yeast chromosomes and nuclear architecture.

Peter Meister1, Lutz R Gehlen, Elisa Varela

  • 1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Methods in Enzymology
|October 16, 2010
PubMed
Summary
This summary is machine-generated.

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Researchers developed new methods to visualize and analyze DNA and protein organization within living yeast cells. These techniques allow detailed study of subnuclear structures and chromatin dynamics.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Understanding the spatial organization of the genome within the nucleus is crucial for regulating gene expression and cellular processes.
  • Existing methods for visualizing specific DNA loci and nuclear proteins in living cells have limitations in resolution and scope.

Purpose of the Study:

  • To establish optimized protocols for tagging genomic DNA sequences in budding yeast.
  • To enable quantitative analysis of subnuclear organization, including DNA locus positioning and chromatin dynamics.
  • To present methods for accurate immunolocalization of nuclear proteins combined with fluorescence in situ hybridization.

Main Methods:

  • Genomic DNA tagging with bacterial operator sites for locus visualization.
  • Quantitative analysis of locus position relative to nuclear center and nuclear pores.

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4D Microscopy of Yeast
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4D Microscopy of Yeast

Published on: April 28, 2019

Related Experiment Videos

Last Updated: Jun 8, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

4D Microscopy of Yeast
12:00

4D Microscopy of Yeast

Published on: April 28, 2019

  • Assessment of chromatin dynamics and relative positioning of tagged loci.
  • Three-dimensional immunolocalization of nuclear proteins.
  • Fluorescence in situ hybridization (FISH) for combined DNA and protein analysis.

    • Main Results:

    • Developed robust protocols for visualizing specific genomic loci in living yeast.
    • Enabled quantitative analysis of subnuclear DNA and protein organization.
    • Facilitated the study of chromatin dynamics and nuclear architecture.
    • Demonstrated the utility of combined immunofluorescence and FISH for high-resolution imaging.

    Conclusions:

    • The described optimized protocols provide a powerful toolkit for robustly analyzing subnuclear organization in intact yeast cells.
    • These methods advance the study of genome organization, chromatin dynamics, and nuclear protein localization.
    • This work offers new possibilities for investigating the relationship between nuclear structure and function.