Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time imaging of transcriptional feedback in nonsense-mediated mRNA decay.

Science advances·2026
Same author

An integrated RNA-centric imaging and omics approach reveals distinct properties and composition of neuronal RNA granules.

bioRxiv : the preprint server for biology·2026
Same author

RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.

Developmental biology·2026
Same author

A series of spontaneously blinking dyes for super-resolution microscopy.

Nature methods·2026
Same author

A platform for analyzing translational control by RBPs at single-mRNA resolution in cells.

Biophysics and physicobiology·2026
Same author

Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth.

Nature communications·2025

Related Experiment Video

Updated: Jun 8, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
07:00

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Published on: June 14, 2013

Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization.

Daniel Zenklusen1, Robert H Singer

  • 1Department of Anatomy and Structural Biology and The Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA.

Methods in Enzymology
|October 16, 2010
PubMed
Summary

This study presents a fluorescent in situ hybridization (FISH) protocol for precisely counting individual messenger RNAs (mRNAs) within single yeast cells. This method enables detailed analysis of gene expression and mRNA metabolism at the single-cell level.

More Related Videos

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
08:18

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

Published on: April 24, 2019

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

Related Experiment Videos

Last Updated: Jun 8, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
07:00

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Published on: June 14, 2013

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
08:18

Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

Published on: April 24, 2019

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Messenger RNA (mRNA) is central to gene expression, linking transcription to translation.
  • Understanding mRNA regulation requires analyzing its spatial and quantitative aspects within single cells.
  • Existing methods may lack the resolution for single mRNA molecule detection in situ.

Purpose of the Study:

  • To develop a robust protocol for detecting and quantifying individual mRNA molecules in single yeast cells.
  • To enable the study of mRNA metabolism, including transcription, processing, localization, and degradation, with spatial resolution.
  • To facilitate absolute quantification of mRNA levels through direct counting.

Main Methods:

  • A stepwise protocol for fluorescent in situ hybridization (FISH) was established.
  • The method was optimized for high-resolution imaging of individual mRNA molecules.
  • Application in single yeast cells to maintain spatial context.

Main Results:

  • Successful detection of individual mRNAs in single yeast cells using the developed FISH protocol.
  • Quantitative analysis of mRNA expression levels through direct counting of molecules.
  • Demonstration of the method's utility for studying various mRNA metabolism aspects.

Conclusions:

  • The described FISH protocol provides a powerful tool for single-cell mRNA analysis in yeast.
  • This method allows for absolute quantification and detailed investigation of mRNA dynamics.
  • It significantly advances the study of gene expression regulation at the subcellular level.