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Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Updated: Jun 8, 2026

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
08:46

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Published on: August 26, 2020

Quantitative high-resolution melting analysis for detecting adulterations.

Eduard Mader1, Joana Ruzicka, Corinna Schmiderer

  • 1Institute for Applied Botany, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Wien, Austria. eduard.mader@vetmeduni.ac.at

Analytical Biochemistry
|October 16, 2010
PubMed
Summary
This summary is machine-generated.

High-resolution melting (HRM) analysis effectively detects plant admixtures in medicinal raw materials, even at very low ratios. This sensitive method, demonstrated with Helleborus niger, also enables precise quantification for quality control.

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Area of Science:

  • Pharmacognosy
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Adulteration of medicinal plant raw materials with other species is a significant challenge.
  • Ensuring the purity and correct identification of plant-based medicines is crucial for safety and efficacy.

Purpose of the Study:

  • To develop and validate sensitive assays for detecting plant admixtures in Helleborus niger using high-resolution melting (HRM) analysis.
  • To demonstrate the capability of HRM for quantifying specific targets within multiplex polymerase chain reaction (PCR) assays.

Main Methods:

  • Development of two exemplary assays utilizing high-resolution melting (HRM) analysis.
  • Application of multiplex PCR combined with HRM for sensitive detection and quantification.
  • Validation of HRM sensitivity for detecting unknown species and Veratrum nigrum admixtures.

Main Results:

  • HRM analysis demonstrated high sensitivity in detecting admixtures, with a detection limit of 1:1000 for unknown species.
  • Exceptional sensitivity was achieved for Veratrum nigrum detection, reaching a ratio of 1:200,000.
  • The study successfully proved HRM's capability for quantification in multiplex PCR, including the use of internal standards.

Conclusions:

  • High-resolution melting (HRM) analysis is a powerful and highly sensitive tool for identifying and quantifying plant admixtures in raw medicinal materials.
  • The developed HRM assays provide a reliable method for quality control, ensuring the purity of Helleborus niger and other medicinal plants.
  • HRM technology offers broad applicability beyond adulteration detection, including the quantification of specific targets using internal standards in molecular assays.