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The extent and effects of peptide sequence scrambling via formation of macrocyclic B ions in model proteins
Irine S Saminathan1, X Simon Wang, Yuzhu Guo
1Department of Chemistry and Centre for Research in Mass Spectrometry, York University, Toronto, Ontario, Canada.
Abstract:
The extent and effects of sequence scrambling in peptide ions during tandem mass spectrometry (MS/MS) have been examined using tryptic peptides from model proteins. Sequence-scrambled b ions appeared in about 35% of 43 tryptic peptides examined under MS/MS conditions. In general, these ions had relatively low abundances with averages of 8% and 16%, depending on the instrumentation used. A few tryptic peptides gave abundant scrambled b ions in MS/MS. However, peptide and protein identifications under proteomic conditions with Mascot were not affected, even for these peptides wherein scrambling was prominent. From the 43 tryptic peptides that have been investigated, the conclusion is that sequence scrambling is unlikely to impact negatively on the accuracy of automated peptide and protein identifications in proteomics.
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