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Lactate in cystic fibrosis sputum
Tobias Bensel1, Martin Stotz, Marianne Borneff-Lipp
1Institute of Hygiene, University Hospital of Halle-Wittenberg, Halle, Germany.
Summary
Lactate levels in cystic fibrosis (CF) sputum decrease with successful antibiotic treatment, indicating neutrophils are the likely source. This suggests lactate can monitor treatment response alongside lung function tests.
Area of Science:
- Biochemistry
- Pulmonology
- Microbiology
Background:
- Antibiotic therapy in cystic fibrosis (CF) aims to improve lung function by reducing neutrophil inflammation.
- The origin and clinical relevance of lactate in the CF lung remain to be fully elucidated.
Purpose of the Study:
- To investigate the source and clinical significance of lactate in CF patients' sputum.
- To determine if lactate levels correlate with treatment response to antibiotics.
Main Methods:
- Lactate measurement in sputum from CF patients (exacerbated and stable) using spectrophotometry and gas chromatography.
- Lung function assessment via spirometry.
- Comparison with control groups including COPD and acute lung inflammation patients.
- Assessment of neutrophil and bacterial lactate production under varying conditions.
Main Results:
- Sputum lactate concentrations significantly decreased after antibiotic therapy in CF patients (3.4±2.3 to 1.4±1.4 mmol/L).
- Improved lung function (FVC increase) correlated with decreased lactate levels.
- Neutrophils were identified as the primary source of lactate in CF sputum, not bacteria.
Conclusions:
- Decreased lactate levels in sputum reflect successful antibiotic therapy in CF patients.
- Lactate may serve as a valuable biomarker for monitoring antibiotic treatment efficacy in CF.
- Neutrophils are the principal contributors to sputum lactate in CF.
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