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Updated: Jun 8, 2026

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Published on: October 25, 2019
Methodological contributions towards LC-MS/MS quantification of free VX in plasma: An innovative approach
Charlotte Debouit1, Alexandre Bazire, Guy Lallement
1I.R.B.A., Antenne C.R.S.S.A., Service de Biospectrométrie, 24 Avenue des Maquis du Grésivaudan, BP 87-F 38702 La Tronche Cedex, France. cdebouit@hotmail.fr
Abstract:
A novel analytical method has been developed to detect and quantify VX (O-ethyl S-(2(diisopropylamino) ethyl) (methylphosphonothioate)) in plasma using an LC-MS/MS technique. VX detection and quantification in plasma following percutaneous exposure represent a formidable challenge and it is an important part of the ongoing struggle against chemical warfare agents. Liquid-liquid extraction of VX from plasma was performed and it generated a recovery rate of approximately 65% followed by an LC-MS/MS analysis in a 100% organic phase. An Allure biphenyl column (Restek) was tested with detection limit at 0.5 pg/mL (5 μL injected). Initial application was focused on human skin grafted on nude mice as an experimental model with proper adjustments done for very small quantities of plasma.
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