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Histones H1 and H4 are present near the replication fork
V Stefanovsky1, S Dimitrov, V Russanova
1Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Molecular Biology Reports
|November 1, 1990
Abstract:
The presence of histones H1 and H4 at the sites of actual DNA synthesis has been studied with Ehrlich ascites tumour cells, pulse labeled for different times with 3H-thymidine and then treated with formaldehyde to crosslink histones to DNA. The fixed chromatin fragments were sonicated to reduce the size of DNA, purified in a CsCl gradient and immunoprecipitated with antibodies to histones H1 and H4. Determination of specific radioactivity in precipitated probes showed that both histones have been associated with nascent DNA even upon 1 min pulse with 3H-thymidine, thus indicating their presence near the replication fork.