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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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APC/C(Cdc20) targets E2F1 for degradation in prometaphase.

Melissa J Peart1, Masha V Poyurovsky, Elizabeth M Kass

  • 1Department of Biological Sciences, Columbia University, New York, NY, USA.

Cell Cycle (Georgetown, Tex.)
|October 16, 2010
PubMed
Summary

The anaphase promoting complex or cyclosome (APC/C) targets E2F1 for degradation during mitosis. APC/C(Cdc20) regulates E2F1 stability, controlling its levels and impacting cell homeostasis.

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Last Updated: Jun 8, 2026

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Published on: January 21, 2012

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • E2F1 activity is tightly regulated, with previous work highlighting Chk1 and Chk2 in its stabilization.
  • Understanding E2F1 protein turnover is crucial for comprehending its role in cell cycle control.

Purpose of the Study:

  • To investigate the mechanisms governing E2F1 protein turnover.
  • To identify the role of the anaphase promoting complex or cyclosome (APC/C) in regulating E2F1 stability.

Main Methods:

  • Investigated E2F1 as a substrate of the APC/C ubiquitin ligase.
  • Examined the effect of APC/C activators (Cdh1, Cdc20) on E2F1 levels.
  • Utilized co-expression of E2F1/DP1 and Cdc20 knockdown/siRNA experiments.
  • Performed cell synchronization studies.

Main Results:

  • E2F1 was identified as a substrate of the APC/C.
  • Ectopic expression of Cdh1 and Cdc20 decreased E2F1 protein levels.
  • The E2F1/DP1 heterodimer was protected from APC/C-mediated degradation.
  • Cdc20 knockdown led to increased and stable E2F1 levels.
  • siRNA against Cdc20 caused E2F1 accumulation in prometaphase cells.

Conclusions:

  • APC/C(Cdc20) targets E2F1 for degradation during early mitosis.
  • This reveals a novel mechanism for controlling free E2F1 levels.
  • Dysregulation of this process may affect cell survival and homeostasis.