Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions

Area of Science:

• Biomedical Engineering
• Tissue Engineering
• Infectious Disease Research

Background:

• Accurate simulation of the native three-dimensional (3D) tissue environment is essential for developing functional in vitro models.
• Understanding host-pathogen interactions requires models that recapitulate in vivo tissue architecture and complexity.

Purpose of the Study:

• To review the use of rotating wall vessel bioreactors for creating advanced 3D in vitro tissue models.
• To discuss the application of these 3D models in the study of infectious diseases.

Main Methods:

• Highlighting the capabilities of rotating wall vessel bioreactors in establishing hierarchical 3D models.
• Describing the range of model complexity, from single-cell to multicellular co-cultures.
• Summarizing the recapitulation of in vivo tissue architecture in engineered models.

Main Results:

• Rotating wall vessel bioreactors enable the creation of sophisticated 3D hierarchical tissue models.
• These models effectively mimic the complex 3D architecture of native tissues.
• The engineered models are applicable to the investigation of various infectious diseases.

Conclusions:

• Rotating wall vessel bioreactors are a valuable tool for engineering 3D in vitro tissue models.
• These advanced models offer significant potential for dissecting host-pathogen interactions in infectious diseases.
• Further application of these bioreactor-based models will advance our understanding of disease mechanisms.