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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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Applications of high-throughput sequencing to chromatin structure and function in mammals

Ian Dunham1

  • 1EMBL-European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton Cambridge CB10 1SD UK. dunham@ebi.ac.uk

F1000 Biology Reports
|October 16, 2010
PubMed

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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...