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Related Concept Videos

Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

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Related Experiment Video

Updated: Jun 8, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Hidden ribozymes in eukaryotic genome sequence.

Sean P Ryder1

  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School 364 Plantation Street, LRB-906, Worcester, MA 01605 USA.

F1000 Biology Reports
|October 16, 2010
PubMed
Summary
This summary is machine-generated.

Self-cleaving ribozymes, RNA enzymes, are common in eukaryotic genomes. These ribozymes may regulate gene expression by catalyzing precise RNA cleavage or ligation within their sequences.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Self-cleaving ribozymes are RNA molecules with catalytic activity.
  • They fold into complex tertiary structures enabling sequence-specific cleavage or ligation.
  • Previously, only a limited number of these ribozymes were known.

Purpose of the Study:

  • To investigate the prevalence of self-cleaving ribozymes in eukaryotic genomes.
  • To explore the potential functions of these ribozymes in gene regulation.

Main Methods:

  • Bioinformatic analysis of eukaryotic genomic sequences.
  • Comparative genomics to identify conserved ribozyme motifs.
  • Functional assays to confirm catalytic activity (details not provided in abstract).

Main Results:

  • Self-cleaving ribozymes are widespread across eukaryotic genomes.
  • Identification of numerous novel ribozyme instances.
  • Evidence suggesting a role in gene expression regulation in certain cases.

Conclusions:

  • Self-cleaving ribozymes are not rare but are a common feature of eukaryotic genomes.
  • These ribozymes have the potential to be involved in the intricate mechanisms of gene expression control.