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Related Experiment Video

Updated: Jun 8, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

A practical guide to constructing and using tissue microarrays.

Ian Chandler1, Richard Houlston, Göran Landberg

  • 1Royal Cancer Hospital Cancer Genetics, Sutton, Surrey, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|October 16, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Tissue microarray (TMA) technology enables high-throughput tissue analysis, saving resources and ensuring reproducibility. Studies show TMA immunohistochemistry is comparable to traditional large sections for prognostic utility.

Area of Science:

  • Biomedical Engineering
  • Pathology
  • Molecular Biology

Background:

  • Tissue microarray (TMA) is a high-throughput tissue analysis method.
  • It allows examination of numerous patient samples efficiently using minimal slides.
  • TMA utilizes formalin-fixed, paraffin-embedded tissues arranged in a grid pattern.

Purpose of the Study:

  • To evaluate the efficiency and reproducibility of Tissue Microarray (TMA) technology.
  • To compare the prognostic utility of TMA-based immunohistochemistry (IHC) with conventional large tissue sections.

Main Methods:

  • Coring tissue cylinders from paraffin blocks.
  • Embedding cores into a recipient paraffin block in a grid pattern.
  • Sectioning the TMA block and applying in situ techniques like IHC.

More Related Videos

SpOT the Correct Tissue Every Time in Multi-tissue Blocks
06:53

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Published on: May 31, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

SpOT the Correct Tissue Every Time in Multi-tissue Blocks
06:53

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Published on: May 31, 2015

Main Results:

  • TMAs enable high-throughput analysis, reducing reagent costs and lab processing time.
  • Sections from TMAs are compatible with standard in situ hybridization and IHC protocols.
  • Immunohistochemical studies demonstrate comparable prognostic utility between TMAs and large tissue sections.

Conclusions:

  • Tissue microarray technology offers a robust, efficient, and reproducible method for tissue analysis.
  • TMAs are a valuable tool for large-scale studies, including prognostic marker evaluation.
  • TMA-based IHC provides results comparable to conventional methods, conserving valuable biological samples.