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Updated: Jun 8, 2026

Preparation of DMMTAV and DMDTAV Using DMAV for Environmental Applications: Synthesis, Purification, and Confirmation
Published on: March 9, 2018
Purification of arsenic (+3 oxidation state) methyltransferase from rat liver cytosol
Zuzana Drobna1, Miroslav Styblo, David J Thomas
1Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Abstract:
Demonstrating the enzymatic basis of arsenic methylation is critical to further studies of the pathway for the conversion of inorganic arsenic into a variety of methylated metabolites. This protocol describes a procedure for the purification of an arsenic methyltransferase from rat liver cytosol. Purification of this enzyme and subsequent cloning of its gene has permitted studies of enzyme structure and function, and has lead to the identification of orthologous genes in genomes of organisms ranging in complexity from sea urchins to humans. These proteins are referred to as arsenic (+3 oxidation state) methyltransferases.
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