Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 8, 2026

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
10:56

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

Porcine skin flow-through diffusion cell system.

R E Baynes1

  • 1North Carolina State University, Raleigh, North Carolina, USA.

Current Protocols in Toxicology
|October 16, 2010
PubMed
Summary

Porcine skin in a diffusion cell system models human skin absorption of topical chemicals. This method accurately assesses chemical penetration rates and extents for dermatological research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short communication: Use of an ultrafiltration device in gland cistern for continuous sampling of healthy and mastitic quarters of lactating cattle for pharmacokinetic modeling.

Journal of dairy science·2018
Same author

Short communication: Effect of 3 phytoceutical products on elimination of bacteria in experimentally induced Streptococcus uberis clinical mastitis.

Journal of dairy science·2018
Same author

Pharmacokinetics and distribution in interstitial and pulmonary epithelial lining fluid of danofloxacin in ruminant and preruminant calves.

Journal of veterinary pharmacology and therapeutics·2016
Same author

The effect of breed and sex on sulfamethazine, enrofloxacin, fenbendazole and flunixin meglumine pharmacokinetic parameters in swine.

Journal of veterinary pharmacology and therapeutics·2014
Same author

Prediction of flunixin tissue residue concentrations in livers from diseased cattle.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2014
Same author

Occurrence of flunixin residues in bovine milk samples from the USA.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2013

Area of Science:

  • Dermatology
  • Toxicology
  • Pharmacokinetics

Background:

  • Percutaneous absorption studies are crucial for evaluating topical product safety and efficacy.
  • Animal models are frequently used to predict human skin absorption.
  • Limitations exist in current animal models for accurately reflecting human skin physiology.

Purpose of the Study:

  • To introduce and validate the use of porcine skin in a flow-through diffusion cell system.
  • To establish a reliable in vitro model for assessing topical chemical absorption.
  • To leverage the anatomical and physiological similarities between porcine and human skin.

Main Methods:

  • Utilizing a flow-through diffusion cell apparatus.
  • Employing excised porcine skin as the biological membrane.
  • Applying chemical formulations topically to the skin surface.
  • Quantifying chemical permeation over time through the diffusion cell.

Main Results:

  • Porcine skin demonstrated a high degree of similarity to human skin in diffusion cell studies.
  • The system allowed for accurate measurement of chemical absorption rates.
  • The model proved effective for evaluating the percutaneous absorption of various topically applied substances.

Conclusions:

  • The porcine skin flow-through diffusion cell system is a valuable in vitro tool for dermatological research.
  • This model provides a more accurate prediction of human skin absorption compared to other animal models.
  • The system facilitates robust safety and efficacy assessments of topical formulations.

More Related Videos

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

Related Experiment Videos

Last Updated: Jun 8, 2026

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
10:56

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022