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Updated: Jun 8, 2026

Gene Trapping Using Gal4 in Zebrafish
13:34

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

zTrap: zebrafish gene trap and enhancer trap database.

Koichi Kawakami1, Gembu Abe, Tokuko Asada

  • 1Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan. kokawaka@lab.nig.ac.jp

BMC Developmental Biology
|October 19, 2010
PubMed
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Researchers developed transgenic zebrafish using Tol2 transposable elements for gene and enhancer trapping. A database, zTrap, aids in finding fish lines with specific GFP and Gal4FF expression patterns for developmental and neurobiology studies.

Area of Science:

  • Genomics
  • Developmental Biology
  • Neurobiology

Background:

  • Developed genetic methods in zebrafish using the Tol2 transposable element for transgenesis, gene trapping, enhancer trapping, and the Gal4FF-UAS system.
  • Created transgenic zebrafish expressing GFP and Gal4FF in specific cells, tissues, and organs via genetic screens.
  • Established a system for manipulating Gal4FF-expressing cells by crossing with UAS effector fish lines, enabling functional studies.

Purpose of the Study:

  • To create a web-based database, zTrap, to enhance the utility of transgenic zebrafish resources.
  • To provide a searchable platform for expression patterns and genomic loci of gene trap and enhancer trap constructs.
  • To facilitate the discovery and utilization of transgenic zebrafish for biological research.

Main Methods:

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Last Updated: Jun 8, 2026

Gene Trapping Using Gal4 in Zebrafish
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Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
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  • Developed gene trap and enhancer trap constructs containing splice acceptors or promoters linked to GFP or Gal4FF.
  • Performed genetic screens to generate transgenic zebrafish with specific expression patterns.
  • Created a web-based database (zTrap) integrating expression data, genomic sequences, and integration site mapping.

Main Results:

  • Generated transgenic zebrafish lines exhibiting GFP and Gal4FF expression in various tissues and organs.
  • Mapped integration sites of trapping constructs onto the Ensembl zebrafish genome.
  • zTrap database provides search functionality for expression patterns and genomic loci, including information on UAS reporter and effector fish.

Conclusions:

  • zTrap serves as a valuable resource for identifying transgenic fish lines with desired expression patterns.
  • Facilitates the discovery of novel genes and cis-regulatory elements by linking insertions to genomic context.
  • Enables comprehensive studies in genomics, developmental biology, and neurobiology using transgenic zebrafish.