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Sensitivity to phages of Streptomyces coelicolor strains harbouring type II restriction endonucleases
J F Aparicio1, C Barbés, C Hardisson
1Department of Functional Biology, University of Oviedo, Spain.
Microbiologia (Madrid, Spain)
|December 1, 1990
Abstract:
The role of type II restriction endonucleases in phage development in two different strains of Streptomyces coelicolor has been analyzed. Two of ten phages tested (phi A4 R4c 1) presented a low efficiency of plating (e.o.p.) in the studied strains. The isolation of host-range mutants of phi A4 and R4c 1, with improved e.o.p. and higher adsorption capability in these two bacterial strains, suggests that the presence of host endonucleases is not the main barrier for these phages, but rather adsorption inability.