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Molecular characterization of the Hepatitis B virus genotypes in Colombia: a Bayesian inference on the genotype F

Mónica Viviana Alvarado Mora1, Camila Malta Romano, Michele Soares Gomes-Gouvêa

  • 1Laboratory of Gastroenterology and Hepatology, São Paulo Institute of Tropical Medicine and Department of Gastroenterology, School of Medicine, University of São Paulo, Brazil. monica.viviana@usp.br

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
|October 19, 2010
PubMed

Insights

Hepatitis B virus (HBV) genotype F, particularly subgenotype F3, is the most common in Colombian blood donors. This study also identified genotypes G and A2, offering insights into HBV's genetic diversity in the region.

Area of Science:

  • * Virology
  • * Epidemiology
  • * Evolutionary Biology

Background:

  • * Hepatitis B virus (HBV) infection is a significant global health concern, with limited understanding of genotype distribution in certain regions like Colombia.
  • * Nine HBV genotypes (A-I) are known, but their geographical prevalence requires further investigation, especially in South America.
  • * Previous studies on HBV genotypes in Colombia are scarce, necessitating detailed characterization of circulating strains.

Purpose of the Study:

  • * To characterize the prevalent Hepatitis B virus genotypes and subgenotypes in a cohort of volunteer blood donors from Colombia.
  • * To analyze the genetic diversity and evolutionary history of HBV strains circulating in Colombia.
  • * To estimate the time of the most recent common ancestor (TMRCA) for HBV genotype F subgenotypes within the Colombian population.

Main Methods:

  • * Amplification and sequencing of a 1306 bp fragment (S/POL regions) from 143 HBsAg-positive blood donors.
  • * Bayesian phylogenetic analysis using Markov Chain Monte Carlo (MCMC) and the maximum clade credibility (MCC) tree approach (BEAST v.1.5.3).
  • * Estimation of TMRCA for HBV/F subgenotypes using two datasets and previously established evolutionary rates.

Main Results:

  • * Genotype F was the predominant HBV genotype (77%), with subgenotypes F3 (75%) and F1b (2%) being most common.
  • * Genotype G (7.7%) and subgenotype A2 (15.3%) were also identified.
  • * Sequence analysis of genotype G suggested multiple introductions into Colombia.
  • * TMRCA was estimated for HBV/F subgenotypes, including Colombian F3 sequences.

Conclusions:

  • * Genotype F, predominantly F3, is the main HBV genotype circulating among blood donors in Colombia.
  • * The presence of genotype G and A2 indicates a diverse HBV genetic landscape in the country.
  • * Phylogenetic analysis provides insights into the evolutionary dynamics and introduction history of HBV genotypes in Colombia.

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