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Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Arboviral Encephalitis01:25

Arboviral Encephalitis

Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

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Related Experiment Video

Updated: Jun 8, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

Recent developments in understanding dengue virus replication.

Silvio Urcuqui-Inchima1, Claudia Patiño, Silvia Torres

  • 1Grupo de Inmunoviología, Sede de Investigación Universitaria, Universidad de Antioquia, Medellín, Colombia.

Advances in Virus Research
|October 19, 2010
PubMed
Summary

Dengue virus, a significant mosquito-borne illness, is increasing globally. Understanding its molecular and cell biology is crucial for developing new control strategies against this potentially fatal disease.

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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Area of Science:

  • Virology
  • Epidemiology
  • Molecular Biology

Background:

  • Dengue is a major mosquito-borne viral disease prevalent in tropical and subtropical areas.
  • Severe dengue hemorrhagic fever and dengue shock syndrome pose significant mortality risks.
  • Epidemiological patterns of dengue are shifting due to vector expansion, travel, and population growth, leading to increased incidence.

Purpose of the Study:

  • To review recent advancements in understanding the molecular and cell biology of the dengue virus life cycle.
  • To provide insights that can aid in designing effective dengue control strategies.
  • To highlight the importance of molecular biology technologies in advancing dengue research.

Main Methods:

  • Review of recently acquired knowledge on dengue virus.
  • Analysis of molecular and cell biology studies.
  • Focus on newly developed molecular biology technologies.

Main Results:

  • Significant progress has been made in understanding the dengue virus life cycle at the molecular and cellular levels.
  • Newly developed molecular biology technologies have been instrumental in these advancements.
  • Current mosquito control methods and lack of vaccines/antivirals necessitate novel approaches.

Conclusions:

  • An in-depth understanding of dengue virus molecular and cell biology is essential for developing new control strategies.
  • Continued research utilizing advanced molecular technologies is vital for combating the increasing global burden of dengue.
  • The review emphasizes the need for innovative approaches beyond traditional vector control and the absence of specific treatments.