Ochrobactrum pecoris sp. nov., isolated from farm animals

Area of Science:

• Microbiology
• Bacteriology
• Taxonomy

Background:

• The genus Ochrobactrum comprises Gram-negative bacteria with diverse environmental and clinical relevance.
• Accurate taxonomic classification is crucial for understanding bacterial roles and interactions.

Purpose of the Study:

• To characterize and classify two novel bacterial isolates from animal sources.
• To determine if the isolates represent a new species within the Ochrobactrum genus.

Main Methods:

• 16S rRNA and recA gene sequencing for phylogenetic analysis.
• Analysis of chemotaxonomic data including quinone systems, polyamines, lipids, and fatty acids.
• DNA-DNA hybridization and phenotypic testing for genotypic and phenotypic differentiation.

Main Results:

• The isolates, 08RB2639(T) and 08RB2781-1, showed high 16S rRNA gene similarity to Ochrobactrum species but distinct recA gene sequences.
• Chemotaxonomic profiles (quinone, polyamines, lipids, fatty acids) aligned with the Ochrobactrum genus.
• DNA-DNA relatedness and phenotypic tests confirmed their distinction from other Ochrobactrum species.

Conclusions:

• The isolates represent a novel species, Ochrobactrum pecoris sp. nov.
• Ochrobactrum pecoris is proposed as a new species, with strain 08RB2639(T) as the type strain.
• This finding expands the known diversity within the Ochrobactrum genus.