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Updated: Jun 8, 2026

Detection and Isolation of Campylobacter spp. from Raw Meat
Published on: February 23, 2024
Antibiotic manipulation of intestinal microbiota to identify microbes associated with Campylobacter jejuni exclusion
A J Scupham1, J A Jones, E A Rettedal
1Pre-Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010, USA. alexandra.scupham@aphis.usda.gov
Abstract:
The ability of various subsets of poultry intestinal microbiota to protect turkeys from colonization by Campylobacter jejuni was investigated. Community subsets were generated in vivo by inoculation of day-old poults with the cecal contents of a Campylobacter-free adult turkey, followed by treatment with one antimicrobial, either virginiamycin, enrofloxacin, neomycin, or vancomycin. The C. jejuni loads of the enrofloxacin-, neomycin-, and vancomycin-derived communities were decreased by 1 log, 2 logs, and 4 logs, respectively. Examination of the constituents of the derived communities via the array-based method oligonucleotide fingerprinting of rRNA genes detected a subtype of Megamonas hypermegale specific to the C. jejuni-suppressive treatments.
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