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Related Experiment Video

Updated: Jun 8, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Tolerance Induction by CD40 Blocking through Specific Antibody in Dendritic Cells.

Mohammad Hossein Karimi¹, Padideh Ebadi, Ali Akbar Pourfathollah

¹Immunology Department, Tarbiat Modares University, Tehran, Iran. pourfa@modares.ac.ir.

Iranian Journal of Allergy, Asthma, and Immunology

|October 19, 2010

Summary

Blocking antibodies targeting CD40 on dendritic cells (DCs) can inhibit T cell activation. This study shows CD40 blockade promotes Th2 cytokine production and reduces IL-12 secretion, highlighting its role in tolerogenic DCs.

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Murine Model of CD40-activation of B cells

Murine Model of CD40-activation of B cells

Published on: March 5, 2010

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Co-stimulatory molecule interactions are crucial for T cell activation.
Blocking antibodies can inhibit specific receptor-ligand interactions.
The CD40 pathway plays a role in T cell stimulation.

Purpose of the Study:

To investigate the effect of a blocking antibody against CD40 on dendritic cell (DC) T cell stimulatory potential.
To determine how CD40 blockade influences cytokine secretion and allostimulatory function.

Main Methods:

Dendritic cells (DCs) were isolated from mouse spleens and cultured in vitro.
A blocking antibody against CD40 (Clone HM40-3) was used, with its titer determined by flow cytometry.
DCs were treated with the antibody and assessed in a mixed lymphocyte reaction (MLR) assay.

Main Results:

CD40 blockade increased IL-4 secretion, promoting Th2 cytokine production by allogeneic T cells.
IL-12 secretion by DCs decreased following CD40 blockade.
DCs with reduced CD40 expression showed diminished responses to alloantigen stimulation in MLR.

Conclusions:

The CD40 pathway is important for the generation of tolerogenic DCs.
Downregulation of CD40 effectively inhibits the allostimulatory function of DCs.
Targeting CD40 offers a strategy for modulating immune responses.