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Production of enterotoxin and cytotoxin by Aeromonas veronii
M S Neves1, M P Nunes, A M Milhomem
1Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brasil.
Abstract:
Fourteen strains of Aeromonas veronii were isolated from salt and fresh water in Rio de Janeiro and investigated for the presence of virulence factors. Eleven (79%) A. veronii strains were positive for enterotoxin by the suckling-mouse test and thirteen (93%) produced hemolysin. Of the 13 A. veronii strains that produced hemolysin, seven were investigated for cytotoxin production using Vero cells as indicator cells and all of them were positive.
Insights
Most Aeromonas veronii strains from Rio de Janeiro possess virulence factors. These bacteria frequently produce enterotoxin, hemolysin, and cytotoxin, indicating potential health risks.
Area of Science:
- Microbiology
- Environmental Science
- Pathogen Research
Background:
- Aeromonas veronii is an opportunistic pathogen found in various aquatic environments.
- Understanding its virulence factors is crucial for assessing public health risks.
Purpose of the Study:
- To investigate the prevalence of virulence factors in Aeromonas veronii strains isolated from Rio de Janeiro's water sources.
- To characterize the enterotoxigenic, hemolytic, and cytotoxic potential of these isolates.
Main Methods:
- Isolation and identification of Aeromonas veronii strains from salt and fresh water samples.
- Detection of enterotoxin using the suckling-mouse assay.
- Assay of hemolysin production.
- Evaluation of cytotoxin activity using Vero cells.
Main Results:
- Eleven out of fourteen (79%) Aeromonas veronii strains tested positive for enterotoxin.
- Thirteen out of fourteen (93%) strains exhibited hemolysin production.
- All seven strains producing hemolysin also showed positive results for cytotoxin production.
Conclusions:
- Aeromonas veronii strains prevalent in Rio de Janeiro waters harbor significant virulence factors.
- The high prevalence of enterotoxin, hemolysin, and cytotoxin suggests a considerable public health concern associated with these bacteria in the region.